dox诱导的shRNA敲低实验中如何设置对照组
在生物医学研究中，RNA干扰（RNAi）技术是探索基因功能的重要工具。基于四环素调控系统（Tet-On/Tet-Off）的dox（多西环素）诱导型shRNA表达体系，因其可时空特异性调控基因表达而备受青睐。然而，这类实验的结论可靠性高度依赖于对照组的合理设计。本文将对dox诱导实验中对照组的科学逻辑进行解析。
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