Principles, Evolution, and Applications of Proximity Labeling
Proximity Labeling (PL) technology utilizes specific enzymes to covalently link labels (such as biotin) to proteins near the target protein, enabling the labeling and subsequent analysis of adjacent proteins. This labeling can be detected by mass spectrometry, revealing interactions between proteins. The advent of this technique has not only provided new tools and perspectives for studying protein-protein interactions but has also greatly advanced our understanding of critical biological processes such as cellular signaling and metabolic regulation.